Lamin Levels In Primary Tumor Cells

Protein expression of lamin a c in du145 cells.

Lamin levels in primary tumor cells. B western blot analysis of lamin b2 protein expression in 20 cases of lung cancer tissues with paired normal lung tissue p 0 01. The nuclear lamina is highly important for migration of both primary and cancer cells. Like many common solid tumor types ovarian cancer of primary tissues or derived cell lines is characterized by aneuploidy or an abnormal and unbalanced number of chromosomes compared to normal diploid cells. Cell migration identified a migration inhibitory role for lamin b1 in lung cancer cells 16 while a migration promoting activity was found for lamin b1 in pancreatic cancer cells 17.

Thus a low lamin a c protein level likely accounts for a deformed nuclear morphology seen in ovarian cancer cells. Moreover a positive association between lamin b1 levels and. Values are normalized to untreated sample. β actin is used as internal control.

We also investigated the expression of several nuclear envelope proteins in a panel of cell lines. 18s rrna levels were used to normalize the lamin a c data. Lamin b2 is overexpressed in nsclc a lmnb2 expression in lung cancer samples from the tcga. Existence of progerin in human cancer cell lines.

Increased lamin levels could help protect cells from mechanical stress caused by the high hydrostatic pressure inside solid tumors. Cell migration requires reposition and reshaping of the cell nucleus. Emerin and lamin b mrna levels were increased in immortalized and cancer cells compared with primary mammary epithelial cells figure 2a and the expression of the emerin protein also increased in breast cancer cells figure 2b. B type lamins are important for proper migration of epicardial cells and neurons and increased lamin b to lamin a ratio accelerates cancer cell migration through confined spaces.

At 48 hours post transfection the cells were analyzed by qrt pcr for lamin a c gene expression levels. Conversely decreasing actomyosin contraction by rock inhibitor y27632 reduced lamin a c level. Cancer cells are highly plastic and heterogeneous in their gene expression so it is likely that different subpopulations of cells with distinct roles in cancer progression exist. Moreover lamin a c was reversibly accumulated at posttranscriptional level under suspension state improving the cell stiffness of reattached breast cancer cells.

Lmnb2 is up regulated in lung adenocarcinoma left and in lung squamous carcinoma right. Similar results have been found for some human cancer cell lines in vitro and in xenografts in vivo 16 20 79 three dimensional xenografts of a human glioblastoma line in stiff mouse subcuta. Lamin a analyses found that increased levels of lamin a inhibit cancer cell migration 18 22 while reduced lamin a levels accelerate cell migration rate 23. Data are means sd n 4.