Lamin C Molecular Weight

The quantification of lamin b bands b reveals a significantly higher level of lamin b proteins in total t and nuclear n protein fractions of u118mg and gli1 cells compared to nha cells c. Ab16048 lmnb1 lamin b1 and ab8245 mouse anti gapdh loading control were incubated overnight at 4 c at 0 1 μg per ml and 1 10000 dilution respectively. During apoptosis lamin a c is specifically cleaved into a large 41 50 kda and a small 28 kda fragment 3 4.

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Lamin A C 4c11 Mouse Mab 4777

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Lamin A C Antibody Cell Signaling Technology

Lamin A Lamin C Antibody 131c3 Nuclear Envelope Marker Ab8984 Abcam

Lamin A C Antibody Nbp2 19324 Novus Biologicals

Lamin B1 Antibody 12987 1 Ap Proteintech

Western blot analysis of extracts from various cell lines using lamin a c 4c11 mouse mab.

Lamin c molecular weight. The change in lmna molecular weight in the mutated hela cells is consistent with an in frame deletion. Lamin a c is cleaved by caspase 6 and serves as a marker for caspase 6 activation. Western blot analysis of extracts from thp 1 cells untreated or treated with cycloheximide chx 10 μg ml overnight followed by tnf α 8902 20 ng. Pbs with 0 1 sodium azide and 50 glycerol ph 7 3.

The prelamin a c maturation pathway includes farnesylation of caax motif zmpste24 face1 mediated cleavage of the last. Lamin a c antibody 636 is a mouse monoclonal igg 2b kappa light chain provided at 200 µg ml. Lamin a is a 664 amino acid protein with a molecular weight of 70 kda calculated 74 139 da and a pi of 7 0 theoretical pi 7 4. Raised against porcine lamin preparation.

Cited in 81 publications. Lamin a consists of a central rod domain flanked by hydrophobic n and c terminal domains. The ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately. Gene id ncbi 4000 conjugate.

Proteolytic cleavage of the c terminal of 18 residues of prelamin a c results in the production of lamin a c. Use a concentration of 1 µg ml. Lamin a c antibody e 1 is a monoclonal anti lamin a c antibody that is recommended for wb ip if ihc p and elisa. The cleavage of lamins results in nuclear dysregulation and cell death 5 6.

65 kda 70 kda. The bands are representative of three independent experiments in triplicate for each protein and beta actin was used as a housekeeping gene. Bands are sharp at the correct molecular weight a bit of nonspecific signal at lower molecular weight but good resolution. Blots were developed with goat anti rabbit igg h l irdye 800cw preadsorbed ab216773 and goat anti mouse igg h l irdye 680rd preadsorbed ab216776 secondary antibodies at 1 10000.

Anti lamin a c antibody 636 is recommended for detection of lamin a and lamin c of mouse rat and human origin by wb ip if ihc p and fcm. Mouse monoclonal clone 4c11 anti lamin a c antibody is used to tag lamins a and or c for detection and quantitation by immunocytochemical and immunohistochemical ihc techniques such as immunoblotting immunoprecipitation and immunofluorescence. 2000 j struct biol 129 313 23.