Lamin B1 Mouse

Reactivity is expected in other species including mouse and rat.

Lamin b1 mouse. Testing by immunofluorescence was validated for human cells. The lamin family of proteins make up the matrix and are highly conserved in evolution. 2019 dec 17 116 51 25870 25879 tss for lmnb1. Lamin b1 antibodies.

The nuclear lamina consists of a two dimensional matrix of proteins located next to the inner nuclear membrane. The blots were probed with anti lamin b1 mouse monoclonal antibody product 33 2000 2µg ml and detected by chemiluminescence using goat anti mouse igg h l superclonal secondary antibody hrp conjµgate product a28177 0 4 µg ml 1 2500 dilution. Anti lamin b1 antibody b 10 is recommended for detection of lamin b1 of mouse rat and human origin by wb ip if and elisa. Lmnb1 mouse p this subsection of the entry information section provides one or more accession number s.

Lamin b1 is a protein that in humans is encoded by the lmnb1 gene. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. When using lamin b1 antibodies as nuclear loading controls be aware that in apoptotic cells lamin b1 is cleaved kottke tj et al. Lamin b1 will also be removed from a nuclear prep if the nuclear membranes are spun out.

Proc natl acad sci u s a. These are stable identifiers and should be used to cite uniprotkb entries. An absence of lamin b1 in migrating neurons causes nuclear membrane ruptures and cell death. During mitosis the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated.

Knockout tested mouse monoclonal lamin b1 antibody 119d5 f1 nuclear envelope marker. Lamin b1 antibody rabbit polyclonal from proteintech validated in western blot wb immunoprecipitation ip immunohistochemistry ihc immunofluorescence if flow cytometry fc chimmunoprecipitation ip enzyme linked immunosorbent assay elisa applications. Lamin b1 and lamin b antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. Lamin b1 119d5 f1 mouse mab recognizes endogenous levels of total lamin b1 by immunofluroescence.

Validated in ihc flow cyt icc if and tested in mouse rat sheep rabbit cow dog human zebrafish. Lamin b1 antibody b 10 is a mouse monoclonal igg 1 kappa light chain provided at 200 µg ml. A 66 kda band corresponding to lamin b1 was observed across all cell lines tested.