Lamin B1 Antibody

Lamin b1 will also be removed from a nuclear prep if the nuclear membranes are spun out. Anti lamin b1 antibody b 10 is recommended for detection of lamin b1 of mouse rat and human origin by wb ip if and elisa. Immunocytochemistry immunofluorescence anti lamin b1 antibody 119d5 f1 nuclear envelope marker ab8982 this image is courtesy of marilena ciciarello patrizia lavia university la sapienza.

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Lamin B1 Antibody Pa5 19468

Lamin B1 Antibody B 10 Scbt Santa Cruz Biotechnology

Human Lamin B1 Antibody Mab8525 100 R D Systems

Anti Lamin B1 Antibody Gtx103292 Genetex

Anti Lamin B1 Antibody Ab65986 Abcam

Lamin B1 Antibody Pa5 32474

This antibody was designed to be a nuclear loading control however it has not yet been tested in appropriate lysates.

Lamin b1 antibody. Specific for an epitope mapping between amino acids 559 586 at the c terminus of lamin b1 of mouse origin. When using lamin b1 antibodies as nuclear loading controls be aware that in apoptotic cells lamin b1 is cleaved kottke tj et al. Anti lamin b1 antibodies are available from several suppliers. Lamin b1 antibody b 10 is a mouse monoclonal igg 1 kappa light chain provided at 200 µg ml.

The cells were fixed in 100 methanol for 6 minutes at 20. In humans this protein is encoded by the gene lmnb1. Methanol fixed hela and 3t3 cells were stained with ab8982 1 50 and 1 20 respectively. Various whole cell extracts 30 μg were separated by 7 5 sds page and the membrane was blotted with lamin b1 antibody gtx103292 diluted by 1 1000.

The reported amino acid length is 586 and the expected mass is 66 4 kda. The protein may also be known as adld lmnb and lmn2. Lamin b1 antibody detects lamin b1 protein by western blot analysis.