Lamin B Molecular Weight

Type b lamins consist of lamin b1 and b2 encoded by separate genes 6 8.

Lamin b molecular weight. Pbs with 0 02 sodium azide and 50 glycerol ph 7 3. Gene id ncbi 4001 conjugate. Our abpromise guarantee covers the use of. Their molecular weight ranges from 60 to 80 kilodaltons kda.

The quantification of lamin b bands b reveals a significantly higher level of lamin b proteins in total t and nuclear n protein fractions of u118mg and gli1 cells compared to nha cells c. When using lamin b1 antibodies as nuclear loading controls be aware that in apoptotic cells lamin b1 is cleaved kottke tj et al. The bands are representative of three independent experiments in triplicate for each protein and beta actin was used as a housekeeping gene. Lamin b1 antibody rabbit polyclonal from proteintech validated in western blot wb immunoprecipitation ip immunohistochemistry ihc immunofluorescence if flow cytometry fc chimmunoprecipitation ip enzyme linked immunosorbent assay elisa applications.

Lamin b1 is also cleaved by caspases during apoptosis 9. Laminin is composed of a laminin alpha 400 kda beta 215 kda and gamma chain 205 kda whose multidomain proteins are encoded by distinct genes lama lamb lamc. Anti lamin b1 antibody b 10 is recommended for detection of lamin b1 of mouse rat and human origin by wb ip if and elisa. Lamin a and c are cleaved by caspases into large 41 50 kda and small 28 kda fragments which can be used as markers for apoptosis 4 5.

In further stages the expression pattern of lamin b 1 decreases and there is a gradual increase in the expression of lamin a. In the latter case as well it is the b type lamins that are expressed in the early stages. This antibody reacts with human mouse rat samples. Currently mammalian species appear to contain at least four major lamins called a b1 b2 and c of which two a and c arise by alternative splicing of the same gene and two minor lamins d and e whose molecular weight varies from species to species.

Lamin b1 and lamin b antibodies are extremely useful as nuclear loading controls for use with nuclear extracts. Broad molecular weight 10 245 kda applications. Specific for an epitope mapping between amino acids 559 586 at the c terminus of lamin b1 of mouse origin. Laminin the most abundant structural and biologically active component in basement membranes is a complex extracellular glycoprotein with high molecular weight 900 kda.

Laminins are high molecular weight 400 to 900 kda proteins of the extracellular matrix they are a major component of the basal lamina one of the layers of the basement membrane a protein network foundation for most cells and organs the laminins are an important and biologically active part of the basal lamina influencing cell differentiation migration and adhesion.