Lamin Ammuno Fluoresence
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Lamin b1 antibody cl3929 nbp2 59783 staining of a 431 cells showing specific staining of nuclear membrane in green.
Lamin ammuno fluoresence. Apart from these regions lamin a and c are identical so that antibodies raised against either protein are likely to. Apart from these regions lamin a and c are identical so that antibodies raised against either protein are likely to cross react with the other. Lamins a and c are alternatively spliced versions of the lmna gene. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope.
The cells were fixed with 4 paraformaldehyde for 10 minutes permeabilized with 0 1 triton x 100 for 10 minutes and blocked with 1 bsa for 1 hour at room temperature. This antibody can also be used in indirect immunofluorescence 1 2 μg ml using hela cells rat nrk and mouse 3t3 cells. These proteins are found in many different cell types in three different forms a b and c. Epub 2010 nov 22 fig 1.
Lamin a has a mass of about 74kda while lamin c is 65kda. Immunocytochemistry immunofluorescence anti lamin b1 antibody nuclear envelope marker ab16048 icc if image of ab16048 stained human hela cells. The cells were methanol fixed 5 min and incubated with the antibody ab16048 1µg ml for 1h at room temperature. Prelamin a c can accelerate smooth muscle cell senescence.
Anti lamin a c terminal antibody is suitable for use in western blot 0 1 0 2 μg ml using hela nuclear extract. Immunocytochemistry immunofluorescence anti pan lamin antibody x67 x167 x233 ab20740 image from mackay dr et al j cell biol. Additionally anti lamin a c terminal antibodies are suitable for use in immunoblotting approx. It acts to disrupt mitosis and induce dna damage in vascular smooth muscle cells vsmcs leading to mitotic.
Immunofluorescence analysis of lamin a was performed using 70 confluent log phase hela cells. Immunocytochemistry immunofluorescence analysis of human lung fibroblasts labeling lamin a with ab26300 at 1 500 dilution. Lamin a c antibodies. The lamin a protein includes a c terminal segment of 98 amino acids missing from lamin c while lamin c has a unique c terminal 6 amino acid peptide not present in lamin a.
Samples were fixed with 3 7 paraformaldehyde for 1 hour and stained for nuclear dna dapi filamentous actin tumor recognition antigen 1 81 and nuclear envelope protein lamin a.
