Lamin A C Protein

Unlike lamin c lamin a is generated in a precursor form called prelamin a.

Lamin a c protein. 2005 identified heterozygosity for the r644c mutation in 4 patients with skeletal and cardiac muscle involvement of varying severity. Two isoforms lamins a and c can be created from this gene via alternative splicing. Lamin a c antibodies. Background lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control dna replication and chromatin.

This creates a high amount of homology between the isoforms. D transfected pc3 and du145 cells were examined for their ability to form colonies in vitro in g418 supplemented selective medium. One major difference between lamins a and c is the absence in lamin c of the caax box. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope.

Lamin a c contains several protein domains. Lmna also known as lamin a c is a protein that in humans is encoded by the lmna gene. Lamin adel10 has a mw of 65 kda and a pi of 8 theoretical pi 8 58. Lamins a and c are alternatively spliced versions of the lmna gene.

Lamin c 572 amino acids differs from lamin a after amino acid 566 containing an alternative 6 amino acid c terminal end vsgsrr. Prelamin a and lamin c differ in structure only at the carboxyl terminus. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding ser22 of human lamin a c protein. Lamin proteins are thought to be involved in nuclear stability chromatin structure and gene expression.

Lamin a c belongs to the lamin family of proteins. It lacks the region encoded by exon 10 resulting in the loss of an acidic and a polyhistidine domain. The authors noted that the mutation is solely within lamin a but not lamin c whereas previously reported mutations causing dilated cardiomyopathy are located more in the rod domain of the protein. Lamin a c protein levels were downregulated up to 2 folds in lncap cells 1 5 folds in du145 cells and 3 folds in pc3 cells respectively.

The lack of lamin a c can be as a novel marker for undifferentiated embryonic stem cells and lamin a c expression is as an early indicator of differentiation pmid. Lamin a and c are identical for the first 566 amino acids but lamin c lacks 98 amino acids at the carboxyl terminus that are present in pre lamin a before post translational processing and contains a unique six amino acid carboxyl terminus figure 1.